LOCUS       40924_34056        3407 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPAL DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Kobayashi H.
  TITLE     Inducible suppression of global translation by overuse of rare 
            codons
  JOURNAL   Appl. Environ. Microbiol. 81 (7), 2544-2553 (2015)
  PUBMED    25636849
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Kobayashi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2015) Contact:Hideki Kobayashi Agency for 
            Marine-Earth Science and Technology, Institute of Biogeosciences; 
            2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL 
            :http://www.jamstec.go.jp/j/
REFERENCE   3  (bases 1 to 3407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2015"; volume: "81"; issue: "7"; pages:
            "2544-2553"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JAN-2015) Contact:Hideki Kobayashi Agency for Marine-Earth 
            Science and Technology, Institute of Biogeosciences; 2-15 
            Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL 
            :http://www.jamstec.go.jp/j/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             200..913
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNGIKV
                     NFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMILLE
                     FVTAAGITHGMDELYK"
     terminator      1127..1213
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1305..1332
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1352..1443
                     /label=AmpR promoter
     CDS             1444..2301
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2334..2428
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2516..3104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3268..3354)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagcttcatg atgaaattcg ccacttgtta gtgtaattcg ctaactcatc ctggcatgtt
       61 gctgttgatt cttcaatcag atctttataa atcaaaaaga taaaaaattg gcacgcaaat
      121 tgtattaaca gttcagcagg acaatcctga acgcagaaat caagaggaca acattaccgg
      181 caggaggttt tttaccggta tgtcaaaggg ggaggagcta ttcacagggg tagtacccat
      241 actagtagag ctagacgggg acgtaaatgg gcaaaagttc tcagtatcag gggaggggga
      301 gggggacgct acatacggga agctaacact aaagttcata tgtacaacag ggaagctacc
      361 cgtaccctgg cccacactag taacaacatt cgggtacggg gtacaatgtt tcgctaggta
      421 ccccgaccac atgaagcaac acgacttctt caagtcagct atgcccgagg ggtacgtaca
      481 agagaggaca atattctaca aggacgacgg gaattacaag acaagggctg aggtaaagtt
      541 cgagggggac acactagtaa ataggataga gctaaagggg atagacttca aggaggacgg
      601 gaatatacta gggcacaaga tggagtacaa ttacaattca cacaatgtat acataatggc
      661 tgacaagccc aagaatggga taaaggtaaa tttcaagata aggcacaata taaaggacgg
      721 gtcagtacaa ctagctgacc actaccaaca aaatacaccc ataggggacg ggcccgtact
      781 actacccgac aatcactacc tatcaacaca atcagctcta tcaaaggacc ccaatgagaa
      841 gagggaccac atgatactac tagagttcgt aacagctgct gggataacac acgggatgga
      901 cgagctatac aagtaggcta gcttggctgt tttggcggat gagagaagat tttcagcctg
      961 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     1021 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1081 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     1141 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1201 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     1261 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     1321 ggatggcctt tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat
     1381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1681 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1981 atgcctacag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2281 gcctcactga ttaagcattg gtaactgtca gaccaagttt acgagctcgc ttggactcct
     2341 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg
     2401 ccgccgggcg ttttttattg gtgagaatcc aagcactagt catgaccaaa atcccttaac
     2461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2881 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
     2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3241 gccgaacgcc taggtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     3301 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     3361 ctagcacgcg taccatggga tcccccgggc tgcaggaatt cgatatc
//
